Bioendo EC Endotoxin Test Kits provide a kind of measurement for quantitative endotoxin testing solution. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored product with maximum absorbance at 405nm. For End-point Chromogenic Endotoxin Test Kit without Diazo Coupling, a regular spectrophotometer or microplate reader is required to read the absorbance at 405nm. The regular spectrophotometer with the function of kinetic experiment, the accuracy of temperature control and 405nm filter, then use that spectrophotometer can read the quantitative endotoxin result of EC kit.